![]() S1 show DII-Venus signals in leaf primordia of additional stages. Stronger DII-Venus signals in the boundary and adjacent adaxial domain indicated weaker auxin signaling input. The abaxial domain in A has pFIL::DsRed-N7 (red) expression. DII-Venus signal is enriched in the adaxial domain from P 2 to P 9. DII-Venus signals are shown in green in A and B, and chlorophyll autofluorescence is in red ( B). ( A and B) Longitudinal and transverse sections through Arabidopsis SAM and leaf primordia region. Transient adaxial low auxin domain is important for leaf polarity patterning. ![]() Opposite to the original proposal, instead of a signal derived from the meristem, we show that a signaling molecule is departing from the primordium to the meristem to promote robustness in leaf patterning.Īuxin leaf polarity meristem stem cell. Our results provide an explanation for the hypothetical meristem-derived leaf polarity signal. Furthermore, we show that auxin flow from emerging leaf primordia to the shoot apical meristem establishes the low auxin zone, and that this auxin flow contributes to leaf polarity. The auxin signal is mediated by the auxin-responsive transcription factor MONOPTEROS (MP), whose constitutive activation in the adaxial domain promotes abaxial cell fate. We also demonstrate that this adaxial low auxin domain contributes to leaf adaxial-abaxial patterning. ![]() Here we show the existence of a transient low auxin zone in the adaxial domain of early leaf primordia. It has long been proposed that the stem cells at the plant shoot apex produce a signal, which promotes leaf adaxial-abaxial (dorsoventral) patterning. 6 Howard Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125 7 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China cells are responsible for organogenesis, but it is largely unknown whether and how information from stem cells acts to direct organ patterning after organ primordia are formed.6 Howard Hughes Medical Institute and Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125 7 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China PMC article.5 Laboratoire de Reproduction et Développement des Plantes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Ecole Normale Supérieure de Lyon, Université Claude Bernard Lyon I, Université de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France.4 Center for Advanced Computing Research, Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125.3 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China and. ![]() 2 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China Howard Hughes Medical Institute and.1 State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100101, China University of Chinese Academy of Sciences, Beijing 100049, China. ![]()
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